Comparative study with the effect of stromal cell derived factor-1 on osteogenic differentiation of human healthy and inflammatory periodontal ligament stem cells / 华西口腔医学杂志

OBJECTIVE@#This study aims to compare the osteogenic differentiation capability of stem cells derived from human inflammatory periodontal ligament tissues (iPDLSCs) with those of stem cells derived from healthy periodontal ligament tissues (hPDLSCs). Both types of tissues were induced by stromal cell derived factor (SDF-1) in vitro.@*METHODS@#iPDLSCs and hPDLSCs were primarily cultured by tissue digestion method and purified by limited dilution cloning. The cells were passaged and identified by stem cell surface marker expression through flow cytometry. Then, we used thiazolyl blue tetrazolium bromide to detect and compare the proliferation capabilities of the iPDLSCs and hPDLSCs. Express of bone volumes were detected by alizarin red staining after SDF-1 was added to the cells. Using alkaline phosphatase, we evaluated the osteogenic differentiation capability of the cells induced by SDF-1. The expression levels of the osteogenesis-related genes of the cells induced by SDF-1 were determined by reverse transcription-polymerase chain reaction.@*RESULTS@#After purification, both iPDLSCs and hPDLSCs expressed stem cell markers. hPDLCSs had a higher proliferation capability than iPDLSCs. Osteogenesis-related genes had higher expression levels in the cells induced by SDF-1 than in those without induction (P<0.05). SDF-1 at 50 and 200 ng·mL⁻¹ concentration greatly affected the differen-tiation capabilities of iPDLSCs and hPDLSCs respectively.@*CONCLUSIONS@#iPDLSCs and hPDLSCs had osteogenic differentia-tion capability. The level of osteogenic differentiation in normal and inflamed periodontal ligament stem cells increases after SDF-1 induction.

Expression of serum IgG4 in patients with different diseases / 北京大学学报(医学版)

Objective:To investigate serum IgG4 levels in different diseases and the changes of serum IgG4 levels in post treatment of IgG4 related disease.Methods:Clinical data of 620 patients who received investigation of serum IgG4 in Peking University People's Hospital from January 1,2015 to March 31,2016 were collected retrospectively.According to the difference of the diseases,they were divided into common group of diseases,autoimmune diseases and IgG4 related diseases;pancreatic disease patients were divided into autoimmune pancreatitis and pancreatic cancer group;According to different treatment stages of the disease,the patients with IgG4 related diseases were divided into pretreatment group and post treatment group.And the expressions of the patients' serum IgG4 levels in different groups were analyzed.Results:The median serum IgG4 level in the group of the patients with common diseases was 0.480 (0.005,50.400) g/L,in the group of autoimmune disease was 0.406 (0.003,18.700) g/L,in the group of IgG4 related diseases was 5.200(0.046,46.000) g/L,which was significantly higher in the group of IgG4 related diseases than the other two groups,and there was obvious statistical significance in serum IgG4 levels between the group of IgG4 related diseases and the other two groups (P ＜0.01);There was no obvious difference in serum IgG4 levels between the common disease group and the autoimmune disease group,and there was no obvious statistical difference in serum IgG4 levels between the two groups (P ＞ 0.05).In the patients with IgG4 related diseases,the median serum IgG4 level in the group of pretreatment patients was 6.540 (1.330,34.100) g/L,and 3.735 (0.063,46.000) g/L in the post treatment patients.Serum IgG4 levels decreased in post treatment group,significantly lower than in pretreatment,there was obvious statistical difference in serum IgG4 levels between the two groups (P ＜ 0.01).The median serum IgG4 level in the group of patients with autoimmune pancreatitis was 3.735 (0.063,46.000) g/L,and 0.438 (0.056,1.130) g/L in the group of patients with pancreatic cancer,which was significantly higher in the group of patients with autoimmune pancreatitis than the others,and there was obvious statistical difference in serum IgG4 levels between the two groups (P ＜0.01).Conclusion:Serum IgG4 levels in patients with different diseases were different,and were significantly higher in patients with autoimmune pancreatitis and IgG4 related diseases,so serum IgG4 levels can provide the basis for the differential diagnosis of different diseases;Serum IgG4 levels in patients with IgG4 related diseases decrease significantly after treatment,so it can be used as an important index to evaluate the curative effect of IgG4 related diseases.

Curative effect of rotary dial up provided bonesetting combined with acupuncture method for treatment of mixed type cervical spondylosis / 新乡医学院学报

Objective To compare the curative effect of rotary dial up provided bonesetting combined with acupuncture method and acupuncture combined with oral medicine for treatment of mixed type cervical spondylosis.Methods Five hundred patients with mixed cervical spondylosis in the Department of Rehabilitation,the Chinese Medicine Hospital of Jiyuan from January 2015 to December 2016 were selected and divided into observation group (n =320) and control group (n =180).The patients in the observation group were treated with rotary dial up provided bonesetting combined with acupuncture method;the patients in the control group were treated with acupuncture and oral medicine.The curative effect of patients in the two groups was observed and analysed by ordered logistics regression.Results The effective rate of patients in the observation group (94.06％,301/320)was significantly higher than that in the control group (83.89％,151/180) (x2 =13.68,P ＜0.01).Ordinal logistics regression results showed that the regression coefficient of treatment methods was 1.38 and the curative effect of treatment group was better.The odds rate of exp(1.380) was 3.975,so effect advantage of the observation group was 3.975 times higher than the control group (P ＜ 0.05).Conclusion The curative effect of rotary dial up provided bonesetting combined with acupuncture method for treatment of mixed type cervical spondylosis is better than acupuncture combined with oral medicine.

Myocardial damage and change of mitochondrial Mn-superoxide dismutase activity in craniocerebral injured rats and the intervening effect of ginkgo biloba extract / 中国中西医结合杂志

<p><b>OBJECTIVE</b>To investigate the myocardial damage and changes of myocardial mitochondrial Mn-superoxide dismutase (Mn-SOD) activity in craniocerebral injured rats and the effect of Ginkgo biloba extract (GBE) on them.</p><p><b>METHODS</b>Craniocerebral injured rats model was established by fluid-percussion and treated with GBE. The dynamical changes of electrocardiograph (ECG) in 24 h were monitored, the serum level of MB isoenzyme of creatine kinase (CK-MB) and the change of myocardial mitochondrial Mn-SOD activity as well as the pathologic changes of myocardium (HE staining) were observed.</p><p><b>RESULTS</b>The occurrence of ECG abnormality obviously increased in the injured rats, accompanied with increased serum CK-MB (P<0.05) and decreased myocardial Mn-SOD levels (P<0.05), and the Mn-SOD activity was negatively correlated with the level of CK-MB (r=-0.997, P<0.05). Pretreatment of GBE resulted in the decrease of ECG abnormality occurrence (P<0.01), serum CK-MB level (P<0.05), and degree of myocardial damage, as well as the increase of Mn-SOD activity in post-craniocerebral injured rats.</p><p><b>CONCLUSIONS</b>Craniocerebral injury can result in distinct myocardial damage, which is possibly correlated with the lowering of anti-oxidation stress level of myocardial cellular mitochondria. GBE possesses the protective effect on myocardial damage after craniocerebral injury.</p>

Recombinant human growth hormone secreted from tissue-engineered bioartificial muscle improves left ventricular function in rat with acute myocardial infarction / 中华医学杂志(英文版)

<p><b>BACKGROUND</b>Experimental studies and preliminary clinical studies have suggested that growth hormone (GH) treatment may improve cardiovascular parameters in chronic heart failure (CHF). Recombinant human GH (rhGH) has been delivered by a recombinant protein, by plasmid DNA, and by genetically engineered cells with different pharmacokinetic and physiological properties. The present study aimed to examine a new method for delivery of rhGH using genetically modified bioartificial muscles (BAMs), and investigate whether the rhGH delivered by this technique improves left ventricular (LV) function in rats with CHF.</p><p><b>METHODS</b>Primary skeletal myoblasts were isolated from several Sprague-Dawley (SD) rats, cultured, purified, and retrovirally transduced to synthesize and secrete human rhGH, and tissue-engineered into implantable BAMs. Ligation of the left coronary artery or sham operation was performed. The rats that underwent ligation were randomly assigned to 2 groups: CHF control group (n = 6) and CHF treatment group (n = 6). The CHF control group received non-rhGH-secreting BAM (GFP-BAMs) transplantation, and the CHF treatment group received rhGH-secreting BAM (GH-BAMs) transplantation. Another group of rats served as the sham operation group, which was also randomly assigned to 2 subgroups: sham control group (n = 6) and sham treatment group (n = 6). The sham control group underwent GFP-BAM transplantation, and the sham treatment group underwent GH-BAM transplantation. GH-BAMs and GFP-BAMs were implanted subcutaneously into syngeneic rats with ligation of the left coronary artery or sham operation was performed. Eight weeks after the treatment, echocardiography was performed. hGH, insulin-like growth factor-1 (IGF-1) and TNF-alpha levels in rat serum were measured by radioimmunoassay and ELISA, and then the rats were killed and ventricular samples were subjected to immunohistochemistry.</p><p><b>RESULTS</b>Primary rat myoblasts were retrovirally transduced to secrete rhGH and tissue-engineered into implantable BAMs containing parallel arrays of postmitotic myofibers. In vitro, they secreted 1 to 2 microg of bioactive rhGH per day. When implanted into syngeneic rat, GH-BAMs secreted and delivered rhGH. Eight weeks after therapy, LV ejection fraction (EF) and fractional shortening (FS) were significantly higher in CHF rats treated with GH-BAMs than in those treated with GFP-BAMs ((65.0 +/- 6.5)% vs (48.1 +/- 6.8)%, P < 0.05), ((41.3 +/- 7.4)% vs (26.5 +/- 7.1)%, P < 0.05). LV end-diastolic dimension (LVEDD) was significantly lower in CHF rats treated with GH-BAM than in CHF rats treated with GFP-BAM (P < 0.05). The levels of serum GH and IGF-1 were increased significantly in both CHF and sham rats treated with GH-BAM. The level of serum TNF-alpha decreased more significantly in the CHF treatment group than in the CHF control group.</p><p><b>CONCLUSIONS</b>rhGH significantly improves LV function and prevents cardiac remodeling in rats with CHF. Genetically modified tissue-engineered bioartificial muscle provides a method delivering recombinant protein for the treatment of heart failure.</p>

Therapeutic effects of recombinant human growth hormone and mesenchymal stem cells transplantation alone or in combination in adriamycin induced cardiomyopathy rat model / 中华心血管病杂志

<p><b>OBJECTIVE</b>To investigate the therapeutic effects of recombinant human growth hormone (rhGH) and mesenchymal stem cells (MSCs) transplantation alone or in combination in adriamycin-induced cardiomyopathy (CM) rat model.</p><p><b>METHODS</b>Wistar rats were divided into normal (n = 6), CM (n = 9), rhGH (2 mg/kg, subcutaneous injection, qod for 1 month, n = 6), MSCs transplantation (MSCs group, 5 x 10(6)/100 microl, n = 7) and rhGH + MSCs (G + M group, n = 7) groups. After 1 month, hemodynamic data, the ratio of heart weight/body weight (HW/BW) and left ventricular weight/body weight (LW/BW) were obtained. The myocardial MSCs marked by BrdU were detected by immunohistochemistry. Cardiac myosin heavy-chain (MHC) and its isozymes were detected by SDS-PAGE technique. Serum growth hormone (GH) and B-type natriuretic peptide (BNP) levels were measured.</p><p><b>RESULTS</b>Compared to CM group, cardiac function remained unchanged in MSC group while significantly improved in rhGH group and further improved in G + M group. HW/BW and LW/BW were similar among groups (all P > 0.05). The MSCs number in the myocardium of G + M group was significantly higher than those of MSCs group. Compared to CM group, V3-MHC was significantly increased in MSCs group (10.33 +/- 0.33 vs 7.43 +/- 2.08, P < 0.05), V1 and V3-MHC were significantly increased in G + M group (86.22 +/- 1.73 vs 77.47 +/- 2.02, 12.44 +/- 0.31 vs 7.43 +/- 2.08, all P < 0.05). GH levels were significantly higher in rhGH and G + M treated animals than that in CM animals [(2.50 +/- 0.68) vs (1.37 +/- 0.09) microg/L, (2.48 +/- 0.90) vs (1.25 +/- 0.42) microg/L, all P < 0.05], BNP levels were significantly lower in rhGH, MSCs and G + M treated groups than that in CM group [(1270.72 +/- 203.72) vs (1462.44 +/- 242.87) ng/L, (1385.00 +/- 250.13) vs ( 1475.29 +/- 281.33) ng/L, (1219.31 +/- 126.71) vs (1451.78 +/- 180.93) ng/L, all P < 0.05].</p><p><b>CONCLUSIONS</b>rhGH but not MSC could improve the cardiac function in adriamycin-induced CM rats. Combined rhGH and MSCs transplantation therapy could further improve cardiac function possibly due to enhanced MSCs growth and transformation into cardiomyocytes.</p>

Recombinant human growth hormone promotes in vitro mesenchymal stem cell growth / 中华心血管病杂志

<p><b>OBJECTIVE</b>To detect the effect of recombinant human growth hormone (rhGH) to mesenchymal stem cells (MSCs) in vitro.</p><p><b>METHODS</b>Various concentrations of rhGH (terminal concentrations 1, 10, 50, 100, 200 and 500 microg/L) were added to medium containing the second generation of MSCs, A value was measured by MTT method at various concentrations and at 24, 48 and 72 h. The expression of insulin-like growth factor 1(IGF-1) mRNA in the presence (24, 48 and 72 h) or absence (the 4(th) day, the 9(th) day, the 10(th) day, the 2(nd) week, the 3(rd) week and the 4(th) week post removal of rhGH) of various rhGH concentrations was determined by RT-PCR.</p><p><b>RESULTS</b>rhGH could promote the MSCs growth at concentration > 10 microg/L in a time-dependent manner. The optimal concentration was 200 microg/L with a growth rate 144.74%. The expression of IGF-1 mRNA increased in a time-dependent manner gradually (0.6749 +/- 0.0084, 0.7781 +/- 0.0068, 0.8230 +/- 0.0060 at 24, 48 and 72 h, respectively at 200 microg/L rhGH). After rhGH withdraw, the expression of IGF-1 mRNA decreased in a time-dependent manner till 2 weeks (0.5287 +/- 0.0077, 0.5747 +/- 0.0050, 0.6068 +/- 0.0056, 0.7071 +/- 0.0089, 0.5791 +/- 0.0057, 0.5781 +/- 0.0081 at the 4(th) day, the 9(th) day, the 10(th) day, the 2(nd) week, the 3(rd) week and the 4(th) week post removal of rhGH).</p><p><b>CONCLUSION</b>rhGH could promote the growth of MSCs in vitro.</p>

A case-control study on the risk factors for road injury / 中华流行病学杂志

<p><b>OBJECTIVE</b>To investigate the risk factors of road injury.</p><p><b>METHODS</b>Case-control study was used. From November 2001 to August 2002, 406 drivers who had 438 drivers who had not experienced a motor vehicle crash in Huanggu district, Shenyang city were recruited by randomly selection on time of day, day of week and site in the same period at same district. Face to face interviews with drivers were conducted according to a highly structured questionnaire covering the circumstances of the current trip, usual behavior and background characteristics of the drivers and the condition of motor vehicles. Stanford sleepiness scale and Epworth sleepiness scale were used to quantify acute and chronic sleepiness respectively.</p><p><b>RESULTS</b>Increased risk was associated with drivers who identified themselves as having chronic doziness (OR = 1.98, 95% CI: 1.26 - 3.12). Increase in risk was associated with measures of acute tiredness, but without statistical significance (OR = 2.38, 95% CI: 0.89 - 6.31). Comparing to permanent daytime work pattern, rotating shifts or permanent night-work pattern increased the risk of crash (OR = 2.09, 95% CI: 1.48 - 2.94). The risk of motor vehicle crash among the drivers who drank alcohol in the previous 6 hours was 3.59 times (95% CI: 1.13 - 11.39) of those drivers who did not drink. Driving violations also contributed to the increased risk of crash (OR = 1.73, 95% CI: 1.22 - 2.46).</p><p><b>CONCLUSION</b>Factors as chronic doziness, rotating shifts or permanent night-work pattern, driving under alcohol impairment, violation of motor vehicle regulation all significantly increased the risk of road injury. Acute sleepiness might serve as a potential risk factor for road injury.</p>

Driver sleepiness and risk of car crashes in Shenyang, a Chinese northeastern city: population-based case-control study / 生物医学与环境科学（英文）

<p><b>OBJECTIVE</b>To estimate the association of driver sleepiness with the risk of car crashes.</p><p><b>METHODS</b>A population-based case-control study was conducted in Shenyang, a northeastern city in China, between November 2001 and July 2002. The case group comprised 406 car drivers involved in crashes, and 438 car drivers recruited at randomly selected sites, and on the day of week, and the time of day when they were driving on highways in the study region during the study period were used as control groups. Face-to-face interviews with drivers were conducted according to a well-structured questionnaire covering the circumstances of their current trip and their background information. Stanford sleepiness scale and Epworth sleepiness scale were used to quantify acute sleepiness and chronic sleepiness respectively.</p><p><b>RESULTS</b>There was a strong association between chronic sleepiness and the risk of car crash. Significantly increased risk of crash was associated with drivers who identified themselves as sleepy (Epworth sleepiness score > or = 10 vs < 10; adjusted odds ratio 2.07, 95% confidence interval 1.30 to 3.29), but no increased risk was associated with measures of acute sleepiness.</p><p><b>CONCLUSIONS</b>Chronic sleepiness in car drivers significantly increases the risk of car crash. Reductions in road traffic injuries may be achieved if fewer people drive when they are sleepy.</p>

Blood lead level and related risk factors among children aged 0-6 years in Beijing / 中华流行病学杂志

<p><b>OBJECTIVE</b>To observe blood lead level and related risk factors among children of 0 - 6-year old in Beijing.</p><p><b>METHODS</b>Stratified-clustered-random sampling and simple random sampling were used. A total of 2 262 children of 0 - 6 years old were investigated from May to July 2001. They were permanent residents in Beijing. Blood lead level was tested by graphite atomizer absorption spectrophotometer. At the same time, related factors were investigated using a standardized questionnaire.</p><p><b>RESULTS</b>The mean lead level of children in Beijing was 96.8 micro g/L with 35.7% of those >/= 100 micro g/L, and 2.5% >/= 200 micro g/L. The proportion of lead poisoning was significantly higher in rural children (P < 0.01). The proportion of lead poisoning in 2-year-old group was higher than that in other age groups. The proportion of lead poisoning in boys was significantly higher than that in girls (P < 0.01). The following factors might serve as major risk factors related to child lead poisoning, such as, never or rarely drinking milk, living in nearby highways (less than 50 meters) or living in the first floor/bungalow, and so on.</p><p><b>CONCLUSION</b>The blood lead level of >/= 100 micro g/L among Beijing children appeared to be a big problem. Decision-makers should pay more attention to prevent blood lead level being high, and to cure these children who suffered in lead poisoning. Effective intervention measures on these target populations should be taken.</p>

Therapeutic value of fosinopril in heart failure / 中国临床药理学与治疗学

Aim To investigate the therapeutic value of fosinopril in heart failure.Methods Thirty SD rats were divided into three groups at random.Rats in heart failure group and fosinopril group were given an 8 weeks overswimming exercise to induce heart failure and the heart failure rats in fosinogril group were treated with fosinopril orally (2 mg?kg?d-1) for 6 weeks.14 weeks later the hemodynamic indexes, myocardiac apoptosis rate, left ventricular mass index (LVMI) and myocardiac interstitial fibrosis were determined.Results The hemodynamic indexes in heart group were remarkably different from those in normal control group, while there was a significant improvement in fosinopril group (P